If you are new to IGB, use this Quick Start Guide get started.
Step 1: Get and start IGB
- Go to BioViz.org and click the Download button
- Select and download the installer for your platform
If you have trouble starting IGB, visit the help page on BioViz.org for assistance.
Step 2: Choose species and genome version
To choose a species and genome version
- Click a shortcut image (loads most recent genome)
- Choose Species and Genome Version using the Current Genome tab.
IGB start screen
If your species or genome version is not listed, you can view it if you have a fasta or 2bit file with sequence data. See Custom Genomes
Step 3. Open data sets
Open data sets from remote data sources (Data Access tab) or by opening local files.
To open a data set from an IGB Quickload data source (see About IGB Quickload)
- Select Data Access
- Select data sets in the Available Data section
To open local files on your computer
- Select File > Open File... or File > Open URL...
- Enter file name or URL
When you select a data sets or a file, IGB adds a new empty track to the main view and lists it in the Data Management table. Empty regions in the new track that do not have data loaded are gray.
IGB window after opening human genome RNA-Seq coverage graphs from Adrenal Gland, Kidney, and Thymus data sets
Step 4: Zoom in
Because many data files contain too much data to view all at once, IGB does not load data into the viewer until you click the Load Data button.
Before loading data, zoom in to a region.
- Click a location in the main view
- Drag the horizontal zoom slider or use plus and minus buttons
IGB horizontal zooming controls
Other ways to zoom
Other ways to zoom include
- Search for a gene by name or keyword (For example, TBATA or thymus)
- Double-click an exon or gene model to zoom in on it
- Click-drag the coordinate axis to zoom in on a region
Step 5: Load data
To load data, click Load Data button. Regions with loaded data show the selected background color; areas without loaded data appear darker.
IGB after loading data
Step 6: Configure tracks
You can reorder the tracks by dragging the Track Label (the Data Management Table reflect changes).
To change style elements of a track, click the track label and use the Annotation or Graph tab to change to change color, track height, annotation label, amount of data shown (stack height), and other options.