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  • Use the + and - tracks separately to run each of these operations.
    • depth (all) (single track)
    • depth (start) (single track)
  • This produces 4 graph tracks.
  • Repeat this by selecting both the - and the + track at the same time (hold shift) and select these single track operations.
  • The results should be identical
    •  Mac
    •  Linux
    •  Windows
  • Select one of the depth all graphs. In the graph panel, Choose Thresholding... . In the thresholding window:
    • Set visibility to On
    • Slide the By Value slider
      • observe the little blocks appear and disappear as the line touches the graph.
        •  Mac
        •  Linux
        •  Windows
      • verify that the values in the graph track correspond to counts of annotations that cover each location
      •  Mac
      •  Linux
      •  Windows
    • Choose Make Track
      • Verify that the new track looks correct.
      •  Mac
      •  Linux
      •  Windows
      • Verify that the new track has an appropriate label (that indicates what threshold was used to create it)
      •  Mac
      •  Linux
      •  Windows
  • See image
  • notice that the depth (start) graphs have data in a 1-base-wide area at the start of the annotations, and where there is only 1 annotation, the graph has a height of 1.

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