Introduction

The Restriction Sites tab maps restriction enzyme sites within the reference genomic sequence.

Restriction Sites tab

How to map sites

To display restriction enzyme sites:

• Click the name of the restriction site you'd like to map in the Restriction Sites tab
  • Select multiple restriction sites by using Ctrl+Click to add them to the list of sites to map on the right
• Click Map Sites
• Click the Clear button to remove all sites.

Viewing results

Mapped sites are shown underneath the coordinate axis in the main view with colors that correspond to those displayed in the Restriction Sites tab. To zoom in on a restriction site, double-click it.

Zoomed-out view of restriction sites recognized by NotI (a rare cutter) mapped onto Chromosome 1 of the Arabidopsis thaliana genome

Zoomed-in view of a single NotI restriction site